RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers.
Angew Chem Int Ed Engl
; 58(5): 1266-1279, 2019 01 28.
Article
em En
| MEDLINE
| ID: mdl-30102012
ABSTRACT
The cellular functions of RNA are not limited to their role as blueprints for protein synthesis. In particular, noncoding RNA, such as, snRNAs, lncRNAs, miRNAs, play important roles. With increasing numbers of RNAs being identified, it is well known that the transcriptome outnumbers the proteome by far. This emphasizes the great importance of functional RNA characterization and the need to further develop tools for these investigations, many of which are still in their infancy. Fluorescent light-up aptamers (FLAPs) are RNA sequences that can bind nontoxic, cell-permeable small-molecule fluorogens and enhance their fluorescence over many orders of magnitude upon binding. FLAPs can be encoded on the DNA level using standard molecular biology tools and are subsequently transcribed into RNA by the cellular machinery, so that they can be used as fluorescent RNA tags (FLAP-tags). In this Minireview, we give a brief overview of the fluorogens that have been developed and their binding RNA aptamers, with a special focus on published crystal structures. A summary of current and future cellular FLAP applications with an emphasis on the study of RNA-RNA and RNA-protein interactions using split-FLAP and Förster resonance energy transfer (FRET) systems is given.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
/
Aptâmeros de Nucleotídeos
/
Fluorescência
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article