Accurate quantification of CXCR4-using HIV-1 variants by Illumina deep-sequencing.
AIDS
; 32(16): 2429-2431, 2018 10 23.
Article
em En
| MEDLINE
| ID: mdl-30102658
ABSTRACT
Next-generation sequencing is a sensitive method for determining HIV-1 tropism but there is a lack of data on the quantification of X4 variants. We evaluated MiSeq and 454 GS-Junior platforms for determining HIV-1 tropism and for quantifying X4 variants. Both platforms were 93% concordant for determining HIV-1 tropism and correlated well for determining the proportion of X4 variants (Spearman correlation, ρâ=â0.748; Pâ<â0.0001). MiSeq Illumina sequencing seems to be well adapted for characterizing X4-containing samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por HIV
/
Receptores de HIV
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HIV-1
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Receptores CXCR4
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Tropismo Viral
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article