Human proteins that interact with RNA/DNA hybrids.
Genome Res
; 28(9): 1405-1414, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-30108179
ABSTRACT
RNA/DNA hybrids form when RNA hybridizes with its template DNA generating a three-stranded structure known as the R-loop. Knowledge of how they form and resolve, as well as their functional roles, is limited. Here, by pull-down assays followed by mass spectrometry, we identified 803 proteins that bind to RNA/DNA hybrids. Because these proteins were identified using in vitro assays, we confirmed that they bind to R-loops in vivo. They include proteins that are involved in a variety of functions, including most steps of RNA processing. The proteins are enriched for K homology (KH) and helicase domains. Among them, more than 300 proteins preferred binding to hybrids than double-stranded DNA. These proteins serve as starting points for mechanistic studies to elucidate what RNA/DNA hybrids regulate and how they are regulated.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
RNA
/
Proteínas de Ligação a RNA
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article