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Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A.
Kitaoku, Yoshihito; Courtade, Gaston; Petrovic, Dejan M; Fukamizo, Tamo; Eijsink, Vincent G H; Aachmann, Finn L.
Afiliação
  • Kitaoku Y; Biochemistry-Electrochemistry Research Unit, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
  • Courtade G; NOBIPOL, Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway.
  • Petrovic DM; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432, Ås, Norway.
  • Fukamizo T; Biochemistry-Electrochemistry Research Unit, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
  • Eijsink VGH; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432, Ås, Norway.
  • Aachmann FL; NOBIPOL, Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway. finn.l.aachmann@ntnu.no.
Biomol NMR Assign ; 12(2): 357-361, 2018 10.
Article em En | MEDLINE | ID: mdl-30117034
ABSTRACT
The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments, as well as an analysis of the secondary structure of the protein based on the secondary chemical shifts.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Apoenzimas / Celulose / Ressonância Magnética Nuclear Biomolecular / Oxigenases de Função Mista Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Apoenzimas / Celulose / Ressonância Magnética Nuclear Biomolecular / Oxigenases de Função Mista Idioma: En Ano de publicação: 2018 Tipo de documento: Article