Rapid detection of Cronobacter spp. in powdered infant formula by thermophilic helicase-dependent isothermal amplification combined with silica-coated magnetic particles separation.
J Immunol Methods
; 462: 54-58, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30144409
ABSTRACT
Cronobacter spp. (previously Enterobacter sakazakii), an opportunistic food-borne pathogen, involved in fatal infections in neonates and infants. Contaminated powdered infant formula (PIF) was considered to be the uppermost infective resource. In the present study, a thermophilic helicase-dependent isothermal amplification (tHDA) based method combined with silica-coated magnetic nanoparticles (Si-MNPs) as a DNA separator for rapid and sensitive detection of Cronobacter has been developed. The whole detection process can be completed in <3â¯h, with a detection limit of 100 and 101â¯CFU/ml Cronobacter in pure culture and artificially contaminated PIF, respectively, with a great sensitivity (94%) and specificity (100%). Hence, this protocol might be useful for screening and monitoring the contamination of Cronobacter spp. in food industry, and helpful for avoiding the economic loss by retard of feedback of the contamination of pathogens in dairy products.
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Base de dados:
MEDLINE
Assunto principal:
DNA Helicases
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Técnicas de Amplificação de Ácido Nucleico
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Fórmulas Infantis
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Nanopartículas de Magnetita
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Cronobacter
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Microbiologia de Alimentos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article