Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical.
Metab Eng
; 51: 99-109, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30144560
ABSTRACT
Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. It was found that use of N-terminal His6-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus could produce 24.5â¯g/L of glutaric acid with low accumulation of l-lysine (1.7â¯g/L), wherein 5-AVA accumulation was not observed during fermentation.
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Base de dados:
MEDLINE
Assunto principal:
Corynebacterium glutamicum
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Ácidos Dicarboxílicos
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Engenharia Metabólica
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Glutaratos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article