Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell.
Analyst
; 143(24): 5912-5917, 2018 Dec 03.
Article
em En
| MEDLINE
| ID: mdl-30191233
ABSTRACT
This work reports the first images obtained by combining an infrared aperture scanning near-field optical microscope (SNOM) with a quantum cascade laser (QCL). The future potential of this set-up is demonstrated by a preliminary study on an OE33 human oesophageal adenocarcinoma cell in which the cell is imaged at 1751 cm-1, 1651 cm-1, 1539 cm-1 and 1242 cm-1. In addition to the 1651 cm-1 image, three other images were acquired within the Amide I band (1689 cm-1, 1675 cm-1 and 1626 cm-1) chosen to correspond to secondary structures of proteins. The four images obtained within the Amide I band show distinct differences demonstrating the potential of this approach to reveal subtle changes in the chemical composition of a cell.
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Base de dados:
MEDLINE
Assunto principal:
Adenocarcinoma
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Células Epiteliais
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Lasers Semicondutores
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Microscopia
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article