An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection.
Chem Commun (Camb)
; 54(77): 10897-10900, 2018 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-30206633
ABSTRACT
Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21. More importantly, the target microRNA-21 could be regenerated with ATP as the fuel rather than a nucleotide segment in conventional approaches, which made the proposed strategy simple and efficient due to the high affinity and strength of the aptamer-target interaction.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Trifosfato de Adenosina
/
Técnicas de Amplificação de Ácido Nucleico
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MicroRNAs
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Aptâmeros de Nucleotídeos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article