Comparison of the Cepheid Xpert Xpress Flu/RSV Assay to in-house Flu/RSV triplex real-time RT-PCR for rapid molecular detection of Influenza A, Influenza B and Respiratory Syncytial Virus in respiratory specimens.
J Med Microbiol
; 67(11): 1576-1580, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-30207514
ABSTRACT
This study compared the performance of the commercially available Xpert Xpress Flu/RSV assay to an in-house FluAB/RSV triplex real-time RT-PCR assay for the detection of influenza A/B viruses and respiratory syncitial virus (RSV) from both nasopharyngeal aspirate (NPA) and nasopharyngeal flocked swab (NPS). A total of 20 external quality assurance (EQA) samples and 172 clinical respiratory samples were tested prospectively using both the Xpert Xpress Flu/RSV assay and the in-house FluAB/RSV triplex assay. For the EQA samples, concordance rate was 100â% when tested with both assays. For clinical samples, there was 100â% agreement between the two assays for detection of influenza A and influenza B, 96.7â% agreement for detection of RSV and 99.7â% agreement for negative results. With a shortened turnaround time and good diagnostic performance, application of the Xpert Xpress Flu/RSV assay can facilitate patient triage for prompt implementation of infection control measures and management of high-risk patients during influenza epidemics.
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MEDLINE
Assunto principal:
Vírus da Influenza A
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Vírus da Influenza B
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Vírus Sinciciais Respiratórios
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Nasofaringe
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Técnicas de Diagnóstico Molecular
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Reação em Cadeia da Polimerase em Tempo Real
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article