Increased Expression of Maturation Promoting Factor Components Speeds Up Meiosis in Oocytes from Aged Females.
Int J Mol Sci
; 19(9)2018 Sep 19.
Article
em En
| MEDLINE
| ID: mdl-30235877
ABSTRACT
The rate of chromosome segregation errors that emerge during meiosis I in the mammalian female germ line are known to increase with maternal age; however, little is known about the underlying molecular mechanism. The objective of this study was to analyze meiotic progression of mouse oocytes in relation to maternal age. Using the mouse as a model system, we analyzed the timing of nuclear envelope breakdown and the morphology of the nuclear lamina of oocytes obtained from young (2 months old) and aged females (12 months old). Oocytes obtained from older females display a significantly faster progression through meiosis I compared to the ones obtained from younger females. Furthermore, in oocytes from aged females, lamin A/C structures exhibit rapid phosphorylation and dissociation. Additionally, we also found an increased abundance of MPF components and increased translation of factors controlling translational activity in the oocytes of aged females. In conclusion, the elevated MPF activity observed in aged female oocytes affects precocious meiotic processes that can multifactorially contribute to chromosomal errors in meiosis I.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Envelhecimento
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Fator Promotor de Maturação
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Meiose
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article