Simultaneous determination of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone in rice bran and vegetable oil samples.
Food Chem
; 271: 630-638, 2019 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-30236726
ABSTRACT
In this study, a simultaneous analytical method of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone were developed using HPLC-DAD-FLD. The developed method allowed the quantification of 18 compounds in 30â¯min. Method validation showed linearity of calibration curves (αâ¯=â¯0.05). RSD of intra-day, inter-day and inter-laboratory precision were less than 4.88%. The limit of detections (LODs) and limit of quantifications (LOQs) were low (0.009-2.166⯵gâ¯g-1) with recoveries around 96.0-102.9%. Results derived from the established method demonstrated a wide variation of detected compounds in rice bran and vegetable oil samples (22.4-1774.6⯵gâ¯g-1 tocols, ND-26484⯵gâ¯g-1 γ-oryzanols, ND-12655⯵gâ¯g-1 phytosterols, ND-3189⯵gâ¯g-1 squalene, ND-105.3⯵gâ¯g-1 cholecalciferol, and ND-54.4⯵gâ¯g-1 phylloquinone). Thus, the developed HPLC-DAD-FLD method is a powerful analytical tool for the above mentioned compounds useful in food and pharmaceutical application.
Palavras-chave
Antioxidants; Campesterol (PubChem CID: 173183); Campesteryl ferulate (PubChem CID: 15056832); Cholecalciferol (PubChem CID: 5280795); Cycloartenyl ferulate (PubChem CID: 5282164); HPLC; Lipids; Oil; Phylloquinone (PubChem CID: 5284607); Rice; Simultaneous analysis; Squalene (PubChem CID: 638072); Stigmasterol (PubChem CID: 5280794); α-Tocopherol (PubChem CID: 14985); α-Tocotrienol (PubChem CID: 5282347); ß-Sitosterol (PubChem CID: 222284); ß-Sitosteryl ferulate (PubChem CID: 9938436); ß-Tocopherol (PubChem CID: 6857447); ß-Tocotrienol (PubChem CID: 5282348); γ-Tocopherol (PubChem CID: 14986); γ-Tocotrienol (PubChem CID: 5282349); δ-Tocopherol (PubChem CID: 92094); δ-Tocotrienol (PubChem CID: 5282350)
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fitosteróis
/
Oryza
/
Óleos de Plantas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article