Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays.
Methods Mol Biol
; 1844: 19-31, 2018.
Article
em En
| MEDLINE
| ID: mdl-30242700
ABSTRACT
RING and U-box ubiquitin ligases promote ubiquitin (Ub) transfer by priming Ub-conjugated E2 in a closed conformation to optimize the thioester bond for nucleophilic attack by substrate lysine. Here, we describe a single-turnover lysine discharge assay for direct assessment of the activity of any RING/U-box E3-E2~Ub complex.
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Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Ubiquitina-Proteína Ligases
/
Lisina
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article