Characterization of T cell immunophenotypes in intestinal transplantation: A pilot study.
Transpl Immunol
; 51: 50-57, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30243797
ABSTRACT
Immunophenotyping of peripheral blood mononuclear cells has been shown to be a useful, non-invasive method of predicting acute cellular rejection (ACR) following intestinal transplantation (ITx). Our objectives were to characterize differences in the T cell immunophenotype of ITx recipients in peripheral blood samples (1) collected late versus early after ITx and (1) associated with episodes of ACR and infectious enteritis. An IRB-approved, cross-sectional study of ITx recipients was performed. Peripheral blood samples were collected during normal visits and episodes of allograft dysfunction. A total of 38 patients were included in the analysis:
31 ITx recipients (87% liver-inclusive allografts) and 7 intestinal failure control patients. Of the ITx patients, 26 patients were pediatric patients (<21â¯years). A total of 70 samples were analyzed from ITx recipients, including 51 during normal visits and 19 during episodes of allograft dysfunction (median of 2 samples per patient; range of 1-6 samples per patient). In the late (nâ¯=â¯32) versus early post-ITx (nâ¯=â¯19) normal samples, there was a significantly higher percentage of central memory CD4 T cells (pâ¯=â¯.001). In the ACR (nâ¯=â¯5) versus infectious enteritis (nâ¯=â¯14) samples, there was a higher percentage of CD8 T cells expressing HLA-DR (pâ¯=â¯.002), CD57 (pâ¯<â¯.001), and KLRG1 (pâ¯<â¯.001) and a higher percentage of CD4 T cells expressing CD57 (pâ¯=â¯.03). Additional studies are needed with larger cohorts to validate these changes in the T cell immunophenotype. Further elucidating T cell immunophenotypes in ITx will lead to a better understanding of immune mechanisms of allograft dysfunction, identification of potential biomarkers in ITx, and optimized selection of immunosuppressive therapies.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T CD4-Positivos
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Transplante de Órgãos
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Linfócitos T CD8-Positivos
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Rejeição de Enxerto
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Intestinos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article