Isolation and characterization of biosurfactant producing and oil degrading Bacillus subtilis MG495086 from formation water of Assam oil reservoir and its suitability for enhanced oil recovery.
Bioresour Technol
; 270: 439-448, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30245313
ABSTRACT
The strains isolated from the formation water were characterized and screened considering their crude oil degradation capability and biosurfactant production ability. The growth kinetics study of isolated Bacillus subtilis MG495086 was carried out by varying growth parameters i.e. carbon source, temperature, pH and salinity. The biosurfactant production was optimized adopting RSM-CCD considering carbon source (1-5%), pH (3-11) and temperature (25-65⯰C) as matrix parameters. The optimum biosurfactant production (6.3⯱â¯0.1â¯g/L) and the minimum surface tension 29.85â¯mN/m were obtained after 96â¯h of incubation under optimal conditions i.e. 3.8% (v/v) of light-paraffin oil as sole carbon source at 62.4⯰C and pH 7.7 with the maximum oil degradation capability of 91.3⯱â¯5%. Critical micelle concentration value of crude biosurfactant was found to be 40â¯mg/L with high emulsification activity of 72.45⯱â¯0.85%. The produced biosurfactant was identified as lipopeptide (Surfactin) and characterized using various analytical techniques to establish its suitability for microbial enhanced oil recovery.
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MEDLINE
Assunto principal:
Bacillus subtilis
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Água
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Campos de Petróleo e Gás
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article