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First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis.
Kaczmarczyk, Agnieszka; Kucharczyk, Halina; Kucharczyk, Marek; Kapusta, Przemyslaw; Sell, Jerzy; Zielinska, Sylwia.
Afiliação
  • Kaczmarczyk A; Department of Genetics and Biosystematics, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland. agnieszka.kaczmarczyk@biol.ug.edu.pl.
  • Kucharczyk H; Department of Zoology, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland.
  • Kucharczyk M; Department of Nature Protection, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland.
  • Kapusta P; Center for Medical Genomics - OMICRON, Jagiellonian University Medical College, Kopernika 7c, 31-034, Kraków, Poland.
  • Sell J; Department of Genetics and Biosystematics, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland.
  • Zielinska S; Department of Bacterial Molecular Genetics, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland.
Sci Rep ; 8(1): 14376, 2018 09 26.
Article em En | MEDLINE | ID: mdl-30258200
Insects' exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial communities of four developmental stages of the fungivorous species Hoplothrips carpathicus (Thysanoptera: Phlaeothripidae), verification of the presence of Wolbachia, in silico prediction of metabolic potentials of the microorganisms, and sequencing its mitochondrial COI barcode. Taxonomy-based analysis indicated that the bacterial community of H. carpathicus contained 21 bacterial phyla. The most abundant phyla were Proteobacteria, Actinobacteria, Bacterioidetes and Firmicutes, and the most abundant classes were Alphaproteobacteria, Actinobacteria, Gammaproteobacteria and Betaproteobacteria, with different proportions in the total share. For pupa and imago (adult) the most abundant genus was Wolbachia, which comprised 69.95% and 56.11% of total bacterial population respectively. Moreover, similarity analysis of bacterial communities showed that changes in microbiome composition are congruent with the successive stages of H. carpathicus development. PICRUSt analysis predicted that each bacterial community should be rich in genes involved in membrane transport, amino acid metabolism, carbohydrate metabolism, replication and repair processes.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Simbiose / Wolbachia / Tisanópteros / Microbiota Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Simbiose / Wolbachia / Tisanópteros / Microbiota Idioma: En Ano de publicação: 2018 Tipo de documento: Article