Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion.
Mater Sci Eng C Mater Biol Appl
; 93: 445-454, 2018 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-30274077
ABSTRACT
Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE). However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells. These deficiencies limit the applicability of these BTE scaffolds to repair bone defects. To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix. We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold. This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model. Healing and mineralization was demonstrated after 12â¯weeks using H&E staining, microcomputerized tomography, and bone mineral density tests. To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects. Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
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MEDLINE
Assunto principal:
Peptídeos
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Regeneração Óssea
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Células da Medula Óssea
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Durapatita
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Quitosana
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Alicerces Teciduais
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Fêmur
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Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article