Brain metabolism under different anesthetic conditions using hyperpolarized [1-13 C]pyruvate and [2-13 C]pyruvate.
NMR Biomed
; 31(12): e4012, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30276897
ABSTRACT
Carbon-13 NMR spectroscopy (13 C MRS) offers the unique capability to measure brain metabolic rates in vivo. Hyperpolarized 13 C reduces the time required to assess brain metabolism from hours to minutes when compared with conventional 13 C MRS. This study investigates metabolism of hyperpolarized [1-13 C]pyruvate and [2-13 C]pyruvate in the rat brain in vivo under various anesthetics pentobarbital, isoflurane, α-chloralose, and morphine. The apparent metabolic rate from pyruvate to lactate modeled using time courses obtained after injection of hyperpolarized [1-13 C]pyruvate was significantly greater for isoflurane than for all other anesthetic conditions, and significantly greater for morphine than for α-chloralose. The apparent metabolic rate from pyruvate to bicarbonate was significantly greater for morphine than for all other anesthetic conditions, and significantly lower for pentobarbital than for α-chloralose. Results show that relative TCA cycle rates determined from hyperpolarized 13 C data are consistent with rates previously measured using conventional 13 C MRS under similar anesthetic conditions, and that using morphine for sedation greatly improves detection of downstream metabolic products compared with other anesthetics.
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MEDLINE
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Encéfalo
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Isótopos de Carbono
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Ácido Pirúvico
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Anestesia
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article