A Clade II-D Fungal Chimeric Diterpene Synthase from Colletotrichum gloeosporioides Produces Dolasta-1(15),8-diene.
Angew Chem Int Ed Engl
; 57(48): 15887-15890, 2018 11 26.
Article
em En
| MEDLINE
| ID: mdl-30277637
ABSTRACT
Based on a terpenoid overproduction platform in yeast for genome mining, a chimeric diterpene synthase from the endophytic fungus Colletotrichum gloeosporioides ES026 was characterized as the (5R,12R,14S)-dolasta-1(15),8-diene synthase. The absolute configuration was independently verified through the use of enantioselectively deuterated terpene precursors, which unequivocally established the predicted C1-III-IV cyclization mode for this first characterized clade II-D enzyme. Extensive isotopic labeling experiments and isolation of the intermediate (1R)-δ-araneosene supported the proposed cyclization mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Alquil e Aril Transferases
/
Colletotrichum
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article