Endoplasmic reticulum stress affected chondrocyte apoptosis in femoral head necrosis induced by glucocorticoid in broilers.
Poult Sci
; 98(3): 1111-1120, 2019 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-30285155
ABSTRACT
In our previous study, chondrocyte apoptosis in femoral head necrosis (FHN)-affected broilers was found to be associated with the endoplasmic reticulum stress (ERS) signaling pathway. In the present study, we further explored the role of ERS-induced chondrocyte apoptosis in FHN-affected broilers and the parallel test was carried out with articular chondrocytes cultivated in vitro. The broilers and chondrocytes were treated with methylprednisolone (MP). The main pathological changes in FHN-affected broilers included the proximal femoral head separated from its articular cartilage and growth plate lesions. MP-treated chondrocytes demonstrated morphology changes, cell viability reduction, secretory capacity dysfunction, and apoptosis. The mRNA expressions of pro-apoptotic genes controlled by ERS signaling pathway were up-regulated both in vivo and in vitro experiments. It showed that MP induced FHN in broilers, activated apoptosis-related genes on ERS signaling pathway, and affected the survival and apoptosis of chondrocytes, and bone growth.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
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Metilprednisolona
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Galinhas
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Apoptose
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Necrose da Cabeça do Fêmur
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Estresse do Retículo Endoplasmático
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Glucocorticoides
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article