The role of αß T-cells in spontaneous regression of melanoma tumors in swine.
Dev Comp Immunol
; 92: 60-68, 2019 03.
Article
em En
| MEDLINE
| ID: mdl-30308209
ABSTRACT
Using a porcine model, we describe Melanoma-Associated CD4+CD8hi T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4+CD8hi phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4+CD8hi TIL have a similar expression of selected markers indicating that they represent effector/memory αß T-cell subset. Moreover, although TIL also contain CD4-CD8+ T-cells, only CD4+CD8hi TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.
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Base de dados:
MEDLINE
Assunto principal:
Suínos
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Linfócitos T
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Linfócitos do Interstício Tumoral
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Melanoma
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Neoplasias Experimentais
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article