Varicella-Zoster Virus in Cerebrospinal Fluid at Relapses of Multiple Sclerosis is Infective in Vitro.
Arch Med Res
; 49(5): 350-355, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-30342846
ABSTRACT
BACKGROUND:
We have reported the presence of varicella-zoster virus (VZV) DNA and viral particles in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients during exacerbation. It is not known whether these viruses are infective.AIM:
To determine whether the VZV found in CSF of MS patients in exacerbation phase are infective.METHODS:
VZV found in CSF of MS patients was quantified by qPCR. Vero E6 cell cultures were incubated with CSF of five MS cases positive for VZV DNA, containing herpes-like viral particles. Propagated virus harvested from these cultures were used to infect new VeroE6 cells. Localization of an immediate-early and a late structural VZV proteins was monitored by confocal microscopy after 72 h. CSF from five non-inflammatory neurological (NIN) patients were used as controls.RESULTS:
A cytopathic effect was found in cultured cells inoculated with CSF from MS patients. Both, structural VZV glycoprotein (gB) and immediate-early VZV protein (IE62) were detected in Vero E6 cultures inoculated with samples from all five MS cases. CSF from control patients produced no effect on Vero E6 cells.CONCLUSION:
When present in the CSF at relapses of MS, VZV is infective under in vitro conditions.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Herpesvirus Humano 3
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Encefalite por Varicela Zoster
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Esclerose Múltipla Recidivante-Remitente
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Efeito Citopatogênico Viral
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article