Low-intensity pulsed ultrasound induces cartilage matrix synthesis and reduced MMP13 expression in chondrocytes.
Biochem Biophys Res Commun
; 506(1): 290-297, 2018 11 17.
Article
em En
| MEDLINE
| ID: mdl-30348521
ABSTRACT
Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics. In previous in vivo and in vitro studies, LIPUS has been shown to have promising effects on cellular elements in articular cartilage, particularly chondrocytes in patients with osteoarthritis. However, the effects of LIPUS on the cellular mechanisms through which LIPUS alters extracellular matrix (ECM) synthesis in chondrocytes are unclear. In this study, we investigated the effects of the optimal intensity and cellular mechanisms of LIPUS on the regeneration of cartilage matrix in chondrocytes. LIPUS induced collagen synthesis and the remodeling of aggrecan via the activation of ERK1/2. In contrast, MMP13 expression was decreased in chondrocytes. Additionally, chondrocytes responded optimally to LIPUS at an intensity higher than the clinical setting for bone fracture healing. These results suggested that LIPUS induced ECM regeneration via increases in hypertrophic chondrocytes and delayed endochondral ossification in chondrocytes.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Cartilagem Articular
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Condrócitos
/
Matriz Extracelular
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Metaloproteinase 13 da Matriz
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Ondas Ultrassônicas
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article