The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.
Nat Methods
; 15(11): 901-904, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30377366
ABSTRACT
We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
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Base de dados:
MEDLINE
Assunto principal:
Conformação Proteica
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Rodopseudomonas
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Síncrotrons
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Cristalografia por Raios X
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Hidrolases
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article