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Components of the Reovirus Capsid Differentially Contribute to Stability.
Snyder, Anthony J; Wang, Joseph Che-Yen; Danthi, Pranav.
Afiliação
  • Snyder AJ; Department of Biology, Indiana University, Bloomington, Indiana, USA.
  • Wang JC; Electron Microscopy Center, Indiana University, Bloomington, Indiana, USA.
  • Danthi P; Department of Biology, Indiana University, Bloomington, Indiana, USA pdanthi@indiana.edu.
J Virol ; 93(2)2019 01 15.
Article em En | MEDLINE | ID: mdl-30381491
ABSTRACT
The mammalian orthoreovirus (reovirus) outer capsid is composed of 200 µ1-σ3 heterohexamers and a maximum of 12 σ1 trimers. During cell entry, σ3 is degraded by luminal or intracellular proteases to generate the infectious subviral particle (ISVP). When ISVP formation is prevented, reovirus fails to establish a productive infection, suggesting proteolytic priming is required for entry. ISVPs are then converted to ISVP*s, which is accompanied by µ1 rearrangements. The µ1 and σ3 proteins confer resistance to inactivating agents; however, neither the impact on capsid properties nor the mechanism (or basis) of inactivation is fully understood. Here, we utilized T1L/T3D M2 and T3D/T1L S4 to investigate the determinants of reovirus stability. Both reassortants encode mismatched subunits. When µ1-σ3 were derived from different strains, virions resembled wild-type particles in structure and protease sensitivity. T1L/T3D M2 and T3D/T1L S4 ISVPs were less thermostable than wild-type ISVPs. In contrast, virions were equally susceptible to heating. Virion associated µ1 adopted an ISVP*-like conformation concurrent with inactivation; σ3 preserves infectivity by preventing µ1 rearrangements. Moreover, thermostability was enhanced by a hyperstable variant of µ1. Unlike the outer capsid, the inner capsid (core) was highly resistant to elevated temperatures. The dual layered architecture allowed for differential sensitivity to inactivating agents.IMPORTANCE Nonenveloped and enveloped viruses are exposed to the environment during transmission to a new host. Protein-protein and/or protein-lipid interactions stabilize the particle and protect the viral genome. Mammalian orthoreovirus (reovirus) is composed of two concentric, protein shells. The µ1 and σ3 proteins form the outer capsid; contacts between neighboring subunits are thought to confer resistance to inactivating agents. We further investigated the determinants of reovirus stability. The outer capsid was disrupted concurrent with the loss of infectivity; virion associated µ1 rearranged into an altered conformation. Heat sensitivity was controlled by σ3; however, particle integrity was enhanced by a single µ1 mutation. In contrast, the inner capsid (core) displayed superior resistance to heating. These findings reveal structural components that differentially contribute to reovirus stability.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reoviridae / Capsídeo / Proteínas do Capsídeo Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reoviridae / Capsídeo / Proteínas do Capsídeo Idioma: En Ano de publicação: 2019 Tipo de documento: Article