A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.
Trans R Soc Trop Med Hyg
; 113(2): 101-104, 2019 02 01.
Article
em En
| MEDLINE
| ID: mdl-30383274
ABSTRACT
Background:
Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.Methods:
Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.Results:
The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.Conclusions:
The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Sangue
/
Cisticercose
/
Cysticercus
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article