Mitochondrial transplantation prolongs cold ischemia time in murine heart transplantation.
J Heart Lung Transplant
; 38(1): 92-99, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30391192
ABSTRACT
BACKGROUND:
Cold ischemia time (CIT) causes ischemiaâreperfusion injury to the mitochondria and detrimentally effects myocardial function and tissue viability. Mitochondrial transplantation replaces damaged mitochondria and enhances myocardial function and tissue viability. Herein we investigated the efficacy of mitochondrial transplantation in enhancing graft function and viability after prolonged CIT.METHODS:
Heterotopic heart transplantation was performed in C57BL/6J mice. Upon heart harvesting from C57BL/6J donors, 0.5 ml of either mitochondria (1â¯×â¯108 in respiration buffer; mitochondria group) or respiration buffer (vehicle group) was delivered antegrade to the coronary arteries via injection to the coronary ostium. The hearts were excised and preserved for 29 ± 0.3 hours in cold saline (4°C). The hearts were then heterotopically transplanted. A second injection of either mitochondria (1â¯×â¯108) or respiration buffer (vehicle) was delivered antegrade to the coronary arteries 5 minutes after transplantation. Grafts were analyzed for 24 hours. Beating score, graft function, and tissue injury were measured.RESULTS:
Beating score, calculated ejection fraction, and shortening fraction were significantly enhanced (p < 0.05), whereas necrosis and neutrophil infiltration were significantly decreased (p < 0.05) in the mitochondria group as compared with the vehicle group at 24 hours of reperfusion. Transmission electron microscopy showed the presence of contraction bands in vehicle but not in mitochondria grafts.CONCLUSIONS:
Mitochondrial transplantation prolongs CIT to 29 hours in the murine heart transplantation model, significantly enhances graft function, and decreases graft tissue injury. Mitochondrial transplantation may provide a means to reduce graft failure and improve transplantation outcomes after prolonged CIT.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Preservação de Órgãos
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Transplante de Coração
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Isquemia Fria
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Mitocôndrias Cardíacas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article