Three-Dimensional Structure of Cytochrome c Nitrite Reductase As Determined by Cryo-Electron Microscopy.

Baymukhametov, T N; Chesnokov, Y M; Pichkur, E B; Boyko, K M; Tikhonova, T V; Myasnikov, A G; Vasiliev, A L; Lipkin, A V; Popov, V O; Kovalchuk, M V

Baymukhametov, T N; Chesnokov, Y M; Pichkur, E B; Boyko, K M; Tikhonova, T V; Myasnikov, A G; Vasiliev, A L; Lipkin, A V; Popov, V O; Kovalchuk, M V.

Afiliação

Baymukhametov TN; National Research Center «Kurchatov Institute¼, Akademika Kurchatova Sqr., 1, Moscow, 123182 , Russia.
Chesnokov YM; National Research Center «Kurchatov Institute¼, Akademika Kurchatova Sqr., 1, Moscow, 123182 , Russia.
Pichkur EB; National Research Center «Kurchatov Institute¼, Akademika Kurchatova Sqr., 1, Moscow, 123182 , Russia.
Boyko KM; National Research Center «Kurchatov Institute¼, Akademika Kurchatova Sqr., 1, Moscow, 123182 , Russia.
Tikhonova TV; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave., 33, bldg. 2, Moscow, 119071, Russia.
Myasnikov AG; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave., 33, bldg. 2, Moscow, 119071, Russia.
Vasiliev AL; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC «Kurchatov Institute¼, 188300 , Leningradskaya Oblast, Gatchina, mkr. Orlova roshcha, 1, Russia.
Lipkin AV; Centre for Integrative Biology, Department of Integrated Structural Biology, Institute of Genetics and of Molecular and Cellular Biology, 67404, 1 rue Laurent Fries, Illkirch, France.
Popov VO; University of California San Francisco Mission Bay, Genentech Hall, San Francisco, CA, 94158- 2517 , USA.
Kovalchuk MV; National Research Center «Kurchatov Institute¼, Akademika Kurchatova Sqr., 1, Moscow, 123182 , Russia.

Acta Naturae ; 10(3): 48-56, 2018.

Article em En | MEDLINE | ID: mdl-30397526

ABSTRACT

ABSTRACT

The structure of cytochrome c nitrite reductase from the bacterium Thioalkalivibrio nitratireducens was determined by cryo-electron microscopy (cryo-EM) at a 2.56 Å resolution. Possible structural heterogeneity of the enzyme was assessed. The backbone and side-chain orientations in the cryo-EM-based model are, in general, similar to those in the high-resolution X-ray diffraction structure of this enzyme.

Palavras-chave

cytochrome c nitrite reductase; high-resolution cryo-electron microscopy; single particle analysis; structural biology

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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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