Microchip gel electrophoretic analysis of perchloric acid-soluble serum proteins in systemic inflammatory disorders.
Electrophoresis
; 40(3): 447-454, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30407655
ABSTRACT
Perchloric acid (PCA) precipitation is a well-known method for the separation of heavily glycosylated proteins and for reducing the masking effect of major serum proteins. The aim of this study is to characterize PCA-soluble serum proteins in healthy individuals and in patients with systemic inflammatory diseases, such as Crohn's disease and sepsis. A PCA precipitation protocol was prepared and adapted to the analytical methods. After PCA treatment of the serum, the soluble proteins in the supernatant were analyzed by SDS-PAGE and by microchip gel electrophoresis (MGE). Characteristic changes of the electrophoretic patterns of the PCA-soluble fractions were observed. Four characteristic bands (at â¼11, â¼65, â¼85, and â¼120 kDa) with varying intensity were detected by MGE. The proportion of the â¼65, â¼85, and â¼120 kDa bands were significantly higher in systemic inflammatory conditions than in healthy individuals (p < 0.001), and characteristic patterns were observed in patients with acute inflammation. The marked differences in the acid-soluble protein patterns, which were observed in patients with ongoing systemic inflammation, might be a good indicator of inflammation. The MGE analysis is a fast screening and quantification method for the detection of characteristic changes among acid-soluble serum proteins.
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Base de dados:
MEDLINE
Assunto principal:
Análise Química do Sangue
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Proteínas Sanguíneas
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Percloratos
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Procedimentos Analíticos em Microchip
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Eletroforese em Gel de Poliacrilamida
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article