Rapid detection of beta-lactamase production including carbapenemase by thin layer chromatography.
J Microbiol Methods
; 156: 15-19, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30468751
ABSTRACT
OBJECTIVES:
To develop a rapid and simple method that can identify the presence of ß-lactamases in clinical isolates and samples, and determine their activity on different types of ß-lactam antibiotics, including carbapenems, within one hour.METHODS:
In this study, we describe a thin layer chromatography-based method for rapid detection of ß-lactamases including carbapenemases. The method relies on the examination of changes in the migration rate of ß-lactams in chromatography, due to degradation by ß-lactamase enzymes. A total of 44 isolates, 29 carbapenemase-producers and 15 non-carbapenemase-producers, were screened by this method.RESULTS:
The method has proven to be able to distinguish ß-lactamases as carbapenemase or non-carbapenemase producing strains with high sensitivity in one hour.CONCLUSIONS:
The method developed, provides information about the production of ß-lactamases by bacteria and ß-lactam drugs inactivated by these enzymes, including carbapenems. This new method may play an important role in guiding antimicrobial treatment, especially in critically ill patients infected bacteria producing ß-lactamases.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Beta-Lactamases
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Cromatografia em Camada Fina
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Acinetobacter baumannii
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Infecções por Enterobacteriaceae
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Escherichia coli
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Enterobacteriáceas Resistentes a Carbapenêmicos
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Klebsiella pneumoniae
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article