Diffusion Profiling of Therapeutic Proteins by Using Solution NMR Spectroscopy.
Chembiochem
; 20(7): 896-899, 2019 04 01.
Article
em En
| MEDLINE
| ID: mdl-30515922
ABSTRACT
Characterizing changes to structure and behavior is an important aspect of therapeutic protein development. NMR spectroscopy is well suited to study interactions and higher-order structure that could impact biological function and safety. We used NMR diffusion methods to describe the overall behavior of proteins in solution by defining a "diffusion profile" that captures the complexities in diffusion behavior. Diffusion profiles offer a simple means to interpret protein solution behavior as a distribution of sizes and association states. As a characterization method, diffusion profiling is well suited to complement and augment traditional biophysical and NMR methods to probe the solution behavior of therapeutic proteins.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Difusão
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article