Monitoring Age-Related Changes in the Lactate/Pyruvate Ratio Using a Colorimetric Assay in a C. elegans Model of Increased Life Span.
Methods Mol Biol
; 1916: 123-132, 2019.
Article
em En
| MEDLINE
| ID: mdl-30535690
ABSTRACT
In the nematode Caenorhabditis elegans (C. elegans), monitoring the lactate/pyruvate ratio in cells helps to detect imbalances in age-related energy metabolism. Here, we describe a modified small-scale extraction in C. elegans and measurement of lactate and pyruvate concentrations using colorimetric assay kits. During sample extraction, protein precipitation is the most critical step for precise determination of intracellular metabolites in C. elegans. Moreover, improved sensitivity and accuracy of colorimetric assay kits contributed to measurements of metabolites in samples derived from small-scale extraction. Using these protocols, we recently detected a metabolic alteration that occurs during aging by the monitoring the lactate/pyruvate ratio in a long-lived mutant of the mammalian tumor suppressor p53 ortholog CEP-1 in C. elegans.
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MEDLINE
Assunto principal:
Proteína Supressora de Tumor p53
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Colorimetria
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Ácido Láctico
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Ácido Pirúvico
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Proteínas de Caenorhabditis elegans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article