A Probe-Enabled Approach for the Selective Isolation and Characterization of Functionally Active Subpopulations in the Gut Microbiome.
J Am Chem Soc
; 141(1): 42-47, 2019 01 09.
Article
em En
| MEDLINE
| ID: mdl-30541282
ABSTRACT
Commensal microorganisms in the mammalian gut play important roles in host health and physiology, but a central challenge remains in achieving a detailed mechanistic understanding of specific microbial contributions to host biochemistry. New function-based approaches are needed that analyze gut microbial function at the molecular level by coupling detection and measurements of in situ biochemical activity with identification of the responsible microbes and enzymes. We developed a platform employing ß-glucuronidase selective activity-based probes to detect, isolate, and identify microbial subpopulations in the gut responsible for this xenobiotic metabolism. We find that metabolic activity of gut microbiota can be plastic and that between individuals and during perturbation, phylogenetically disparate populations can provide ß-glucuronidase activity. Our work links biochemical activity with molecular-scale resolution without relying on genomic inference.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
/
Microbioma Gastrointestinal
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article