Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine.
Int J Mol Sci
; 19(12)2018 Dec 12.
Article
em En
| MEDLINE
| ID: mdl-30545076
ABSTRACT
BACKGROUND:
Antimicrobial peptides (AMP) play a pivotal role in innate host defense and in immune response. The delineation of new MS-based profiling tools, which are able to produce panels of AMP of the nasal fluid (NF), may be attractive for the discovery of new potential diagnostic markers of respiratory disorders.METHODS:
Swabs collected NF from healthy patients and from patients with respiratory disorders. We used a fast procedure based on mesoporous silica particles (MPS) to enrich NF in its AMP component in combination with MALDI-TOF/TOF MS as a key tool for rapidly analyzing clinical samples.RESULTS:
Reproducible MS peptide fingerprints were generated for each subject and several AMP were detected including (Human Neutrophil Peptides) HNPs, Statherin, Thymosin-ß4, Peptide P-D, II-2, ß-MSP, SLPI, Lysozyme-C, and their proteo-forms. In particular, Statherin, Thymosin-ß4, and Peptide P-D were accurately identified by direct MS/MS sequencing. Examples of applicability of this tool are shown. AMP fingerprints were obtained before and after a nasal polypectomy as well as before and post-treatment with azelastine/fluticasone in one case of allergic rhinitis.CONCLUSION:
The potential of our platform to be implemented by new mesoporous materials for capturing a wider picture of AMP might offer an amazing opportunity for diagnostic clinical studies on individual and population scales.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Líquidos Corporais
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Mapeamento de Peptídeos
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Nariz
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Dióxido de Silício
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Peptídeos Catiônicos Antimicrobianos
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Medicina de Precisão
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article