Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites.
Biochim Biophys Acta Mol Cell Res
; 1866(3): 349-359, 2019 03.
Article
em En
| MEDLINE
| ID: mdl-30595161
ABSTRACT
Using electron and fluorescence microscopy techniques, we identified various physical contacts between peroxisomes and other cell organelles in the yeast Hansenula polymorpha. In exponential glucose-grown cells, which typically contain a single small peroxisome, contacts were only observed with the endoplasmic reticulum and the plasma membrane. Here we focus on a novel peroxisome-vacuole contact site that is formed when glucose-grown cells are shifted to methanol containing media, conditions that induce strong peroxisome development. At these conditions, the small peroxisomes rapidly increase in size, a phenomenon that is paralleled by the formation of distinct intimate contacts with the vacuole. Localization studies showed that the peroxin Pex3 accumulated in patches at the peroxisome-vacuole contact sites. In wild-type cells growing exponentially on medium containing glucose, peroxisome-vacuole contact sites were never observed. However, upon overproduction of Pex3 peroxisomes also associated to vacuoles at these growth conditions. Our observations strongly suggest a role for Pex3 in the formation of a novel peroxisome-vacuole contact site. This contact likely plays a role in membrane growth as it is formed solely at conditions of strong peroxisome expansion.
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MEDLINE
Assunto principal:
Pichia
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Peroxissomos
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Proteínas de Saccharomyces cerevisiae
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Peroxinas
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Proteínas de Membrana
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article