Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds.
Microscopy (Oxf)
; 68(1): 92-97, 2019 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-30608618
ABSTRACT
X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.
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Base de dados:
MEDLINE
Assunto principal:
Sementes
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Arabidopsis
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Microtomografia por Raio-X
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article