Identification of pregnancy-associated glycoprotein family (PAG) in the brown bear (Ursus arctos L.).

ABSTRACT

Pregnancy-associated glycoproteins (PAGs) are abundant embryo-originated products expressed in the pre-placental trophoblast and, later, in the post-implantational chorionic epithelium of some mammalian species. This paper describes the identification and cellular immunolocalization of the chorionic PAG family in the discoidal-type placenta of the brown bear (Ursus arctos L. - Ua), in which the PAGs were named 'UaPAG-Ls'. The study used 1) Western blot for total placental glycoproteins; and 2) cross-species heterologous double fluorescent immunohistochemistry (IHC) for cellular immune-localization of the PAGs. This is the first study reporting the identification and immunolocalization of the UaPAG-L family in placental cells during early pregnancy in the brown bear. Our Western analysis revealed a dominant mature 72 kDa UaPAG-L isoform was expressed in all Ua placentas during early pregnancy. Various other UaPAG-L isoforms (16-66 kDa) were also identified. Using IHC, the UaPAG-L proteins were localized to trophectodermal cells (TRD), where signal intensity resembled intense TRD proliferation within developing placenta. The data increases our general knowledge of PAG proteins localized in discoidal-type placenta during early pregnancy in the brown bear.