Metagenomic engineering of the mammalian gut microbiome in situ.
Nat Methods
; 16(2): 167-170, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30643213
ABSTRACT
Engineering of microbial communities in open environments remains challenging. Here we describe a platform used to identify and modify genetically tractable mammalian microbiota by engineering community-wide horizontal gene transfer events in situ. With this approach, we demonstrate that diverse taxa in the mouse gut microbiome can be modified directly with a desired genetic payload. In situ microbiome engineering in living animals allows novel capabilities to be introduced into established communities in their native milieu.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia de Proteínas
/
Metagenômica
/
Microbiota
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Microbioma Gastrointestinal
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article