Patterns of Dendritic Basal Field Orientation of Pyramidal Neurons in the Rat Somatosensory Cortex.

ABSTRACT

The study of neuronal dendritic orientation is of interest because it is related to how neurons grow dendrites to establish the synaptic input that neurons receive. The dendritic orientations of neurons in the nervous system vary, ranging from rather heterogeneously distributed (asymmetric) to homogeneously distributed (symmetric) dendritic arbors. Here, we analyze the dendritic orientation of the basal dendrites of intracellularly labeled pyramidal neurons from horizontal sections of Layers II-VI of the hindlimb somatosensory (S1HL) cortex of 14-d-old (P14) rats. We used circular statistics and proposed two new graphical descriptive representations of the neuron. We found that the dendritic pattern of most neurons was asymmetric. Furthermore, we found that there is a mixture of different types of orientations within any given group of neurons in any cortical layer. In addition, we investigated whether dendritic orientation was related to the physical location within the brain with respect to the anterior, dorsal, posterior and ventral directions. Generally, there was a preference towards the anterior orientation. A comparison between layers revealed that the preference for the anterior orientation was more pronounced in neurons located in Layers II, III, IV, and Va than for the neurons located in Layers Vb and VI. The dorsal orientation was the least preferred orientation in all layers, except for Layers IV and Va, where the ventral orientation had the lowest preference. Therefore, the orientation of basal dendritic arbors of pyramidal cells is variable and asymmetric, although a majority has a single orientation with a preference for the anterior direction in P14 rats.