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Microbe-focused glycan array screening platform.
Geissner, Andreas; Reinhardt, Anika; Rademacher, Christoph; Johannssen, Timo; Monteiro, João; Lepenies, Bernd; Thépaut, Michel; Fieschi, Franck; Mrázková, Jana; Wimmerova, Michaela; Schuhmacher, Frank; Götze, Sebastian; Grünstein, Dan; Guo, Xiaoqiang; Hahm, Heung Sik; Kandasamy, Jeyakumar; Leonori, Daniele; Martin, Christopher E; Parameswarappa, Sharavathi G; Pasari, Sandip; Schlegel, Mark K; Tanaka, Hidenori; Xiao, Guozhi; Yang, You; Pereira, Claney L; Anish, Chakkumkal; Seeberger, Peter H.
Afiliação
  • Geissner A; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Reinhardt A; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Rademacher C; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Johannssen T; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Monteiro J; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Lepenies B; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Thépaut M; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Fieschi F; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Mrázková J; Immunology Unit and Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
  • Wimmerova M; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Schuhmacher F; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Götze S; Immunology Unit and Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
  • Grünstein D; Université Grenoble Alpes, Institut de Biologie Structurale, F-38044 Grenoble, France.
  • Guo X; CNRS, Institut de Biologie Structurale, F-38044 Grenoble, France.
  • Hahm HS; Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Biologie Structurale, F-38044 Grenoble, France.
  • Kandasamy J; Université Grenoble Alpes, Institut de Biologie Structurale, F-38044 Grenoble, France.
  • Leonori D; CNRS, Institut de Biologie Structurale, F-38044 Grenoble, France.
  • Martin CE; Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Biologie Structurale, F-38044 Grenoble, France.
  • Parameswarappa SG; Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
  • Pasari S; Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
  • Schlegel MK; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Tanaka H; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Xiao G; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Yang Y; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Pereira CL; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
  • Anish C; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
  • Seeberger PH; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Proc Natl Acad Sci U S A ; 116(6): 1958-1967, 2019 02 05.
Article em En | MEDLINE | ID: mdl-30670663
ABSTRACT
Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Proteínas de Transporte / Análise em Microsséries Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Proteínas de Transporte / Análise em Microsséries Idioma: En Ano de publicação: 2019 Tipo de documento: Article