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A Human Skin Model Recapitulates Systemic Sclerosis Dermal Fibrosis and Identifies COL22A1 as a TGFß Early Response Gene that Mediates Fibroblast to Myofibroblast Transition.
Watanabe, Tomoya; Baker Frost, DeAnna; Mlakar, Logan; Heywood, Jonathan; da Silveira, Willian A; Hardiman, Gary; Feghali-Bostwick, Carol.
Afiliação
  • Watanabe T; Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, 96 Jonathan Lucas St, MSC 637, Charleston, SC 29425, USA. nabetomo0968@yahoo.co.jp.
  • Baker Frost DA; Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, 96 Jonathan Lucas St, MSC 637, Charleston, SC 29425, USA. bakerde@musc.edu.
  • Mlakar L; Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, 96 Jonathan Lucas St, MSC 637, Charleston, SC 29425, USA. logan.mlakar@gmail.com.
  • Heywood J; Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, 96 Jonathan Lucas St, MSC 637, Charleston, SC 29425, USA. heywood.jonathan@gmail.com.
  • da Silveira WA; Center for Genomic Medicine, Bioinformatics, Medical University of South Carolina, Charleston, SC 29425, USA. willian.abraham@gmail.com.
  • Hardiman G; Center for Genomic Medicine, Bioinformatics, Medical University of South Carolina, Charleston, SC 29425, USA. G.Hardiman@qub.ac.uk.
  • Feghali-Bostwick C; Departments of Medicine and Public Health Sciences, Medical University of South Carolina, Charleston, SC 29425, USA. G.Hardiman@qub.ac.uk.
Genes (Basel) ; 10(2)2019 01 22.
Article em En | MEDLINE | ID: mdl-30678304
ABSTRACT
Systemic sclerosis (SSc) is a complex multi-system autoimmune disease characterized by immune dysregulation, vasculopathy, and organ fibrosis. Skin fibrosis causes high morbidity and impaired quality of life in affected individuals. Animal models do not fully recapitulate the human disease. Thus, there is a critical need to identify ex vivo models for the dermal fibrosis characteristic of SSc. We identified genes regulated by the pro-fibrotic factor TGFß in human skin maintained in organ culture. The molecular signature of human skin overlapped with that which was identified in SSc patient biopsies, suggesting that this model recapitulates the dermal fibrosis characteristic of the human disease. We further characterized the regulation and functional impact of a previously unreported gene in the setting of dermal fibrosis, COL22A1, and show that silencing COL22A1 significantly reduced TGFß-induced ACTA2 expression. COL22A1 expression was significantly increased in dermal fibroblasts from patients with SSc. In summary, we identified the molecular fingerprint of TGFß in human skin and demonstrated that COL22A1 is associated with the pathogenesis of fibrosis in SSc as an early response gene that may have important implications for fibroblast activation. Further, this model will provide a critical tool with direct relevance to human disease to facilitate the assessment of potential therapies for fibrosis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escleroderma Sistêmico / Colágeno / Miofibroblastos Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escleroderma Sistêmico / Colágeno / Miofibroblastos Idioma: En Ano de publicação: 2019 Tipo de documento: Article