Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system.
J Genet Eng Biotechnol
; 16(2): 639-643, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30733783
ABSTRACT
Gynura procumbens (Lour.) Merris one of medicinal plant which was carried out used as antioxidant, anticancer, anti-inflammatory, hepatoprotective, and antimicrobial. Many strategies were used to increase the production of biomass and valuable compounds. This study was to investigate the variation effect of growth regulators and immersion frequency on production of biomass and flavonoid contained of G. procumbens shoots culture in temporary immersion bioreactor. Stem nodes were used as an explants and induction of shoots were done in solid MS medium supplemented with many kinds of growth regulator. The best treatments were used to produce biomass and flavonoid compounds in temporary immersion bioreactor; there are combination of IAA 2â¯mg/L and BA 4, 6, 8â¯mg/L and immersion frequency (5 min each 3â¯h; 15â¯min each 12â¯h). Results showed that the growths of G. procumbens shoots in solid MS medium were influenced by supplementation of growth regulators. MS medium supplemented with single cytokinine (6 mg/L kinetin) and combination of auxin (IAA) and cytokinine (BA) caused increasing of shoots growth. Production of biomass of G. procumbens in temporary immersion bioreactor was achieved in long immersion interval (12â¯h) and highest flavonoid production was obtained in combination treatment of immersion frequency 15â¯min each 12â¯h and MS medium supplemented with IAA 2â¯mg/L, BA 8â¯mg/L.
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MEDLINE
Idioma:
En
Ano de publicação:
2018
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Article