In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells.
J Vis Exp
; (143)2019 01 20.
Article
em En
| MEDLINE
| ID: mdl-30735200
ABSTRACT
Plasma cells (PCs) secrete large amounts of antibodies and develop from B cells that have been activated. PCs are rare cells located in the bone marrow or mucosa and ensure humoral immunity. Due to their low frequency and location, the study of PCs is difficult in human. We reported a B to PC in vitro differentiation model using selected combinations of cytokines and activation molecules that allow to reproduce the sequential cell differentiation occurring in vivo. In this in vitro model, memory B cells (MBCs) will differentiate into pre-plasmablasts (prePBs), plasmablasts (PBs), early PCs and finally, into long-lived PCs, with a phenotype close to their counterparts in healthy individuals. We also built an open access bioinformatics tools to analyze the most prominent information from GEP data related to PC differentiation. These resources can be used to study human B to PC differentiation and in the current study, we investigated the gene expression regulation of epigenetic factors during human B to PC differentiation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmócitos
/
Linfócitos B
/
Linfopoese
/
Memória Imunológica
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article