Efficient production of androstenedione by repeated batch fermentation in waste cooking oil media through regulating NAD+/NADH ratio and strengthening cell vitality of Mycobacterium neoaurum.
Bioresour Technol
; 279: 209-217, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30735930
ABSTRACT
The bioprocess for producing androstenedione (AD) from phytosterols by using Mycobacterium neoaurum is hindered by nicotinamide adenine dinucleotides (NAD+ and NADH) ratio imbalance, insoluble substrate, and lengthy biotransformation period. This study aims to improve the efficiency of AD production through a combined application of cofactor, solvent, and fermentation engineering technologies. Through the enhanced type II NADH dehydrogenase (NDH-II), the NAD+/NADH ratio and ATP levels increased; the release of reactive oxygen species decreased by 42.32%, and the cell viability improved by 54.17%. In surfactant-waste cooking oil-water media, the conversion of phytosterol increased from 23.92% to 94.98%. Repeated batch culture successfully reduced the biotransformation period from 30 to 17â¯days, the productivity was 13.75 times more than the parent strain. This study is the first to improve the productivity of AD by enhancing NDH-II and provides a new strategy to increase the accumulation of NAD+-dependent metabolites during biotransformation.
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Base de dados:
MEDLINE
Assunto principal:
Óleos
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Culinária
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Fermentação
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Androstenodiona
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Mycobacterium
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NAD
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article