A "Green" Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in Croton Crassifolius.
Molecules
; 24(4)2019 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-30769949
ABSTRACT
Clerodane diterpenoids are the main bioactive constituents of Croton crassifolius and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) process combined with a sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLCâ»MS) technique together for the rapid extraction and determination of clerodane diterpenoids in C. crassifolius. All calibration curves showed good linearity (r > 0.9943) within the test ranges and the intra- and inter-day precisions and repeatability were all within required limits. This modified HGEâ»UHPLCâ»MS method only took 5 min to extract nine clerodane diterpenoids in C. crassifolius and another 12 min to quantify these components. The results indicated that the quantitative analysis based on UHPLCâ»MS was a feasible method for QC of clerodane diterpenoids in C. crassifolius, and the findings outlined in the current study also inferred the potential of the method in the QC of clerodane diterpenoids in other complex species of plants.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Extratos Vegetais
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Cromatografia Líquida de Alta Pressão
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Croton
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Diterpenos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article