Depression of Fungal Polygalacturonase Activity in Solanum lycopersicum Contributes to Antagonistic Yeast-Mediated Fruit Immunity to Botrytis.
J Agric Food Chem
; 67(12): 3293-3304, 2019 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-30785743
ABSTRACT
The acquisition of susceptibility to necrotrophy over the course of ripening is one of the critical factors limiting shelf life. In this study, phytopathology and molecular biology were employed to explore the roles of pectinase in fruit susceptibility and ripening. Solanum lycopersicum fruit softened dramatically from entirely green to 50% red, which was accompanied by a continuously high expressed SlPG2 gene. The necrotrophic fungus Botrytis cinerea further activated the expression of SlPGs and SlPMEs to accelerate cell wall disassembly, while most of the polygalacturonase inhibitor proteins encoding genes expression were postponed in ripe fruit following the pathogen attack. Pectin induced the antagonistic yeast to secrete pectinolytic enzymes to increase fruit resistance against gray mold. The activities of pathogenic pectinase of B. cinerea were correspondingly depressed in the pectin-inducible yeast enzyme elicited ripe fruit. These data suggest that pectinase is a molecular target for regulation of disease resistance during fruit ripening.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Poligalacturonase
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Leveduras
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Proteínas Fúngicas
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Solanum lycopersicum
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Botrytis
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Antibiose
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article