The Association Between the Transforming Growth Factor Beta-1 -509C>T Gene Polymorphism and Primary Open Angle Glaucoma in North Eastern Iran.
Rep Biochem Mol Biol
; 7(2): 167-173, 2019 Jan.
Article
em En
| MEDLINE
| ID: mdl-30805396
ABSTRACT
BACKGROUND:
Glaucoma is a common cause of irreversible blindness. Transforming growth factor beta-1(TGF-ß1) is the main isoform of TGF-ß superfamily in the eye. Overexpression of TGF-ß1 is shown to be related with the glaucoma. Studies have shown that the presence of mutant T allele of TGF-ß1 -509C>T polymorphism (rs1800469) is associated with increased gene expression. So, in present study, association of the TGF-ß1-509C>T gene polymorphism and primary open angle glaucoma (POAG) in patients from north east of Iran was investigated.METHODS:
A case-control study was conducted on 112 POAG patients and 112 control participants. TGF-ß1- 509C>T genotyping was done by PCR-restriction fragment length polymorphism (PCR-RFLP) method using Bsu36I restriction enzyme. Moreover, cup to disk ratio(CDR), intraocular pressure (IOP) and visual acuity (VA) were measured. The obtained results were statistically analyzed.RESULTS:
The highest frequency of genotype in the control group was related to CC genotype (44.6%), but the heterozygous CT genotype (45.6%) was observed as the highest frequency of genotypes in patient group (P value 0.022, OR for TT genotype 2.54 CI95% for OR 1.22, 5.27). Also, the frequency of the T mutant allele showed a significant difference between case and control groups (P value 0.005, OR 1.73 CI95% for OR 1.18, 2.53).CONCLUSION:
In conclusion, a significant association was seen between TGF-ß1 -509C>T gene polymorphism and POAG disease and inheritance of mutant T allele is considered to be a risk factor for glaucoma in patients living in North Eastern part of Iran.
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MEDLINE
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article