Diagnostic Potential of Anti-RTE30 Polyclonal Antibodies In A Blocking Elisa For Toxocara canis Detection.
J Parasitol
; 105(1): 64-68, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30807721
ABSTRACT
The main etiologic agent of human toxocariasis, a zoonotic disease, is the helminth Toxocara canis. Among the diagnostics used for human toxocariasis, ELISA using T. canis excretion and secretion antigen (TES) is considered as a standard technique. TES antigen requires the cultivation of T. canis larvae, which makes its production difficult. Besides this, the use of TES antigen does not eliminate the cross-reactions with other similar proteins that are produced by other intestinal worms. In this context, recombinant antigens are being tested to improve the diagnosis of human toxocariasis. Herein, we describe the production of polyclonal antibodies against recombinant protein TES30 (pAb-rTES30) and evaluate its use in a blocking ELISA (b-ELISA) using human sera. The b-ELISA showed 95.6% sensitivity and 94.4% specificity. Thus, the b-ELISA using pAb-rTES30 offers a viable option for toxocariasis diagnosis owing to its configuration, which prevents cross-reactivity with non-species-specific antibodies.
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Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
/
Toxocaríase
/
Toxocara canis
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Antígenos de Helmintos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article