Dietary Radix Bupleuri extracts improves hepatic lipid accumulation and immune response of hybrid grouper (Epinephelus lanceolatusâ × Epinephelus fuscoguttatusâ).
Fish Shellfish Immunol
; 88: 496-507, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30826414
ABSTRACT
In this study, two experiments were performed to explore the effect of Radix Bupleuri extracts (RBE) on growth, lipid deposition and metabolism and immune response of hybrid grouper (Epinephelus lanceolatusââ¯×â¯Epinephelus fuscoguttatusâ) using in vitro and in vivo models. In vitro, we used 2â¯ml/L 20% lipid emulsion (LE)-induced steatosis in hybrid grouper primary hepatocytes, then RBE (200, 400 and 800⯵g/ml) was added to the hepatocytes after (post-treatment) the incubation with 20% LE (2â¯ml/L) in the culture medium. We found that RBE markedly increased cell viability, which were consistent with hepatocytes morphological structure examination and lipid metabolism and immune related genes study. The above result suggested that RBE has a protective effect on this model of hepatocytes damage. In vivo, five graded levels of RBE at 0, 200, 400, 800 and 1600â¯mg/kg diet were supplemented to a basal diet with 15% lipid levels (high lipid), and fed to a total of 300 hybrid grouper with an average initial weight of 25.58⯱â¯0.05â¯g for 8 weeks. Growth performance, liver histology, plasma biochemical parameters, and expression of genes involved in lipid metabolism and immune-related were measured. The study indicated that dietary RBE significantly improved growth performance and feed utilization and reduced hepatosomatic index. Dietary supplementation with 200-800â¯mg/kg RBE diets effectively decreased serum ALP, ALT, AST and LDH contents in fish. Furthermore, adipogenesis relative mRNA levels of DGAT2, G6PD, ME1 and DGKα in fish fed 200-400â¯mg/kg RBE diets were lower (Pâ¯<â¯0.05) than in those fed RBE0 diets, while dietary supplementation with 200-800â¯mg/kg RBE diets up-regulated lipolysis-related genes (CPT1, LPL and PPARα) expression in the liver of hybrid grouper. Moreover, dietary RBE down-regulated the expression of apoptosis-related genes (caspase-9), up-regulated the expression of antioxidant genes (CAT) and immune-related genes (MHC2, IKKα and TGF-ß1). Thus, our data suggest that RBE suppressed lipid accumulation and enhanced immune capability in hybrid grouper both in vitro and in vivo. These results offer new insight into RBE as a hepatoprotective in fish.
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MEDLINE
Assunto principal:
Bass
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Extratos Vegetais
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Suplementos Nutricionais
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Hepatócitos
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Metabolismo dos Lipídeos
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Fígado Gorduroso
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article