Quantitative analysis of tetrahydrofolate metabolites from clostridium autoethanogenum.
Metabolomics
; 14(3): 35, 2018 02 16.
Article
em En
| MEDLINE
| ID: mdl-30830344
ABSTRACT
INTRODUCTION:
Quantification of tetrahydrofolates (THFs), important metabolites in the Wood-Ljungdahl pathway (WLP) of acetogens, is challenging given their sensitivity to oxygen.OBJECTIVE:
To develop a simple anaerobic protocol to enable reliable THFs quantification from bioreactors.METHODS:
Anaerobic cultures were mixed with anaerobic acetonitrile for extraction. Targeted LC-MS/MS was used for quantification.RESULTS:
Tetrahydrofolates can only be quantified if sampled anaerobically. THF levels showed a strong correlation to acetyl-CoA, the end product of the WLP.CONCLUSION:
Our method is useful for relative quantification of THFs across different growth conditions. Absolute quantification of THFs requires the use of labelled standards.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tetra-Hidrofolatos
/
Clostridium
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article