MiR-182-5p inhibited proliferation and metastasis of colorectal cancer by targeting MTDH.
Eur Rev Med Pharmacol Sci
; 23(4): 1494-1501, 2019 Feb.
Article
em En
| MEDLINE
| ID: mdl-30840271
ABSTRACT
OBJECTIVE:
To investigate the role and molecular mechanism of miR-182-5p in the development of colorectal cancer (CRC), thereby providing a theoretical basis for new CRC therapeutic targets. PATIENTS ANDMETHODS:
The expression of miR-182-5p in CRC clinical cases and biological cell lines was detected. On-line target gene prediction and Luciferase reporter gene assay were performed to screen and verify the target of miR-182-5p, respectively. The role of miR-182-5p in CRC cell function was further analyzed.RESULTS:
MiR-182-5p expression was significantly decreased in both CRC tissues and cell lines. Metadherin (MTDH) was screened and verified as a functional target of miR-182-5p. The cell proliferation, invasion and migration ability of CRC cells were significantly inhibited after the up-regulation of miR-182-5p. However, MTDH limited the anti-cancer effects of miR-182-5p in CRC cells.CONCLUSIONS:
Our research demonstrated the inhibitory function of miR-182-5p in CRC. Therefore, the miR-182-5p/MTDH axis was expected to be one of the targets of CRC targeted therapy.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
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Proteínas de Ligação a RNA
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MicroRNAs
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Proliferação de Células
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Proteínas de Membrana
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article